mRNA translation · real-time
Open platform · v1.0
Design better
mRNA faster

AI-powered mRNA sequence design agent. Codon optimization, UTR engineering, miRNA avoidance, and secondary structure prediction — all cell-type aware, built on open-source science.

Launch agent Read the docs

Free to use · No credit card required

26M+
UTR sequences indexed
7
Cell-type RBP profiles
6
Agentic tool calls
3
Ranked candidates per run
100%
Open-source data stack
See it in action
From protein sequence to
optimized mRNA in 60 seconds

Watch the agent validate your ORF, optimize codons with tissue-specific HIVE-CUT tables, select UTRs, predict secondary structure, scan for miRNA seeds, and synthesize 3 ranked candidate constructs.

Design Parameters
Gene
Anti-CD19 BBz CAR
ORF
ATGCTTCTCCTGGTGACAAGCCTTCTGCTCTGT…1428 nt
Cell type
T cell Dendritic
Chemistry
m1Ψ · CleanCap · A120
CpG depleted
Agent log
Sequence
Analysis
Candidates
Results
60 sec
From ORF to 3 ranked candidates
6 tools
Deterministic scientific pipeline
Cell-aware
HIVE-CUT tissue-matched optimization
Try it free →
Capabilities
Every layer of mRNA design,
in one agent

RNAV8 reasons across codon tables, UTR libraries, miRNA atlases, and structure predictors simultaneously — then synthesises ranked candidates with full scientific rationale.

⬡
Codon optimization
Tissue-matched HIVE-CUT tables with 5′ ramp, CpG depletion, and CAI maximization. Codon bias matched to your exact delivery cell type.
HIVE-CUT 5′ ramp CpG depleted CAI scoring
◈
UTR engineering
Curated library of validated 5′ and 3′ UTRs — HBB, VEEV, Xenopus Hsp70, AES chimeric — scored by cell type, Kozak strength, and expression boost data.
UTRdb 2.0 Kozak scoring Expression boost
◎
Structure prediction
ViennaRNA MFE calculation of the 5′ region. Flags structured hairpins that impair 43S ribosome scanning and suggests synonymous fixes.
ViennaRNA MFE −4 threshold Window scan
⌁
miRNA avoidance
7-mer seed scanning against cell-type–specific miRNA panels from FANTOM5 and miRNATissueAtlas2025. Auto-resolves hits with single-base UTR edits.
FANTOM5 miRBase v22 373 tissues
⦿
RBP binding analysis
ATtRACT-based motif scanning for HuR, TTP, CPEB, and MBNL1. Stabilizing and destabilizing sites scored per cell type for accurate half-life estimation.
ATtRACT HuR · TTP · CPEB Half-life model
▲
Multi-candidate synthesis
Three ranked constructs per run: balanced, immune-evasion (max CpG depletion), and stability-focus. Composite scores with full scientific rationale.
3 candidates Composite scoring PMID citations
How it works
6-tool agentic
pipeline

The LLM orchestrator plans which tools to call, reasons over their outputs, resolves conflicts, and synthesises candidates — all in a single run. Each tool is a deterministic scientific function; the agent is the reasoning layer.

Full pipeline docs →
1
validate_orf
ATG start, stop codon, internal stops, cryptic GT-AG splice sites, uORF risk
2
optimize_codons
Tissue HIVE-CUT table · 5′ ramp · CpG depletion · CAI calculation
3
select_utrs
Score UTRdb library by cell type · Kozak strength · expression boost · uORF status
4
predict_structure
ViennaRNA MFE of 5′ region · must be >−4.0 kcal/mol · window scan
5
scan_mirna_rbp
7-mer seed scan · FANTOM5 cell-type miRNAs · ATtRACT RBP motifs
6
synthesize_candidates
3 ranked constructs: balanced / immune-evasion / stability-focus
Open data
Built on open science

Every data source is publicly accessible and citable. No black boxes.

database
UTRdb 2.0
26M eukaryotic UTRs · miRNA targets (TarBase v8) · uORF annotations · Rfam motifs
database
HIVE-CUT
Tissue-specific human codon usage tables from GTEx mRNA-seq · 12 tissues
database
miRBase v22
Mature miRNA sequences · 2,654 human miRNAs · seed regions for scanning
atlas
FANTOM5
Cell-type miRNA expression profiles · 492 sRNA libraries · primary human cells
atlas
miRNATissueAtlas2025
61,593 samples · 373 tissue types · most comprehensive miRNA expression resource
database
ATtRACT
370 human RBPs · 1,858 PWMs · HuR, TTP, CPEB, MBNL1 motif scanning
tool
ViennaRNA
Gold-standard MFE RNA secondary structure prediction · RNAfold · LinearFold
your data
RNAV8bio repos
Connect your private GitHub to layer proprietary UTR and codon data on top
Open-source core. The entire computational pipeline — codon optimizer, miRNA scanner, RBP scorer — is open source and auditable. More features coming soon — batch runs, API access, and more.
View on GitHub
Start designing
better mRNA today

Free to use. No credit card. Your first design in under 60 seconds.

Launch the agent Read the docs